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KMID : 0861020090240030161
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Korea Journal of Herbology
2009 Volume.24 No. 3 p.161 ~ p.167
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Anti-carcinogenetic and Anti-metastatic Effects of Extract from Maekmoondong-tang in HepG2 Cells
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Cheon Myeong-Sook
Chun Jin-Mi
Yoon Tae-Sook
Lee A-Young
Moon Byeong-Cheol
Choo Byung-Kil
Kim Seong-Hwan
Kim Ho-Kyoung
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Abstract
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Objectives : Maekmoondong-tang (MMDT), a Korean herbal medicine, has been used to treat severe dry cough in patients with bronchitis and pharyngitis. MMDT has been reported to have anti-inflammatory, anti-allergic, immunomodulatory, secretory-modulating, and metabolic regulatory actions. However, there are no evidence in regard to the effects of MMDT on carcinogenesis and metastasis. Here, we investigated the effects of 70% ethanol extract of MMDT on cell viability, apoptosis, and motility in human hepatocarcinoma HepG2 cells.
Methods : Cell viability was measured using the CCK-8 assay, and the apoptosis induction was evaluated by caspase-3 activity. To detect apoptotic features, the cells treated with MMDT were stained with 4’-6-diamidino-2-phenylindole (DAPI). Cell motility was examined by Boyden chamber assay and Real-time Cell Index of Migration assay. Gelatin zymography also performed to measure matrix metalloproteinase (MMP)-2/9 activity.
Results : We found that MMDT significantly inhibited cell proliferation and increased caspase-3 activity in a dose-dependent manner in HepG2 cells. Apoptotic features such as chromatin condensation and apoptotic bodies were observed in MMDT-treated cells by DAPI staining. MMDT also suppressed PMA-induced cell motility and activities of MMP-2/9.
Conclusions : Our results exhibited that MMDT possess the anti-carcinogenetic and anti-metastatic activities via caspase-3 activation and down-regulation of cell motility and invasion in HepG2 cells. Therefore, these findings suggest that MMDT could be potentially applied to the prevention and treatment of cancer.
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KEYWORD
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Anti-tumor, Apoptosis, Caspase-3, Maekmoondong-tang, Metastasis, MMPs
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